Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Epigenetic Control of Translation Checkpoint and Tumor Progression via RUVBL1-EEF1A1 Axis.

doi: 10.1002/advs.202206584

Figure Lengend Snippet: Figure 6. Targeting RUVBL1 synergizes with JQ1 in EwS. A) EwS xenograft tumor volume and B) tumor growth fold (day 15 vs day 7; n = 12 tumor sites per group) in control (gray), JQ1 (green), sgRUVBL1 (blue), and combination (red) groups. C) Chemical structure of CB-6644 and the effect of CB-6644 treatment on the proliferation of A673 cells (n = 4 for each group). D) Western blot of H4K8ac, H4K12ac, EEF1A1, histone H4, and GAPDH in A673 cells treated with vehicle (DMSO) and CB-6644 (0.5 × 10−6 m). E) RT-qPCR of EEF1A1 mRNA in A673-Cas9 cells treated with DMSO and 0.5 × 10−6 m CB-6644 (n = 3 for each group). F) Flow cytometric profiles of HPG labeled (cyan) compared to the nonlabeled (gray) cells in A673 cultures incubated

Article Snippet: After one week, mice were treated daily with CB-6644 (75 mg kg−1 d−1; MedKoo Biosciences), JQ1 (40 mg kg−1 d−1; S7110, Selleckchem), or vehicle (30% Solutol HS15/PBS) through intraperitoneal injection.

Techniques: Control, Western Blot, Quantitative RT-PCR, Labeling, Incubation